TGF-β1 induces ILK expression in renal tubular epithelial cells.
(a–d) Western blot analyses show that
TGF-β1 induced ILK protein expression in a time- and dose-dependent
manner. HKC cells were incubated with either the same concentration of
TGF-β1 (2 ng/ml) for various periods of time as indicated
(a and c) or increasing amounts of TGF-β1
for 24 hours (b and d). Cell lysates were
immunoblotted with Ab’s against ILK and actin, respectively.
(a and b) Representative Western blots.
(c and d) Graphic presentation of relative ILK
abundance (fold induction) normalized to actin. Data are presented as mean
± SEM of three independent experiments. (e and
f) Immunofluorescence staining shows the localization of ILK in
control (e) or TGF-β1–treated HKC cells
(f). Arrowheads indicate positive ILK staining. Scale bar: 5